Journal: Bioactive Materials

Article Title: Hypoxia preconditioned MSC exosomes attenuate high-altitude cerebral edema via the miR-125a-5p/RTEF-1 axis to protect vascular endothelial cells

doi: 10.1016/j.bioactmat.2025.06.018

Figure Lengend Snippet: Effect of H-EXO on functional recovery of vascular endothelial cells after hypoxia. (A) Schematic diagram of transwell cell permeability assay (created with biorender.com ). (B) Lower chamber fluorescence over time. (C), (D) Representative pictures and statistical analysis of in vitro wound healing test results. (E), (F) DCFH-DA staining was used to detect intracellular ROS production and statistical analysis. (G), (H) Expression and statistical analysis of RTEF-1, VEGF, P2Y12, and INOS were investigated by WB. All data are presented as mean ± SD, statistical analysis using one-way analysis of variance. ∗ P < 0.05, ∗∗ P < 0.01, ∗ versus Hypo group, # versus N-EXO-Hypo group. Scale bar: 200 μm (C).

Article Snippet: The primary antibodies included GAPDH (Sequoia Golden Bridge, TA-08, China), RTEF-1 (Abcam, AB133533 , UK), VEGF (Cell Signaling Technology, 9698s, USA), INOS (Thermo Fisher Scientific, PA1-036, USA), and P2Y12 (Thermo Fisher Scientific, 702516, USA).

Techniques: Functional Assay, Permeability, Fluorescence, In Vitro, Staining, Expressing

Journal: Bioactive Materials

Article Title: Hypoxia preconditioned MSC exosomes attenuate high-altitude cerebral edema via the miR-125a-5p/RTEF-1 axis to protect vascular endothelial cells

doi: 10.1016/j.bioactmat.2025.06.018

Figure Lengend Snippet: RTEF-1 as a target gene of miR-125a-5p in H-EXO alleviating hypoxia-induced vascular endothelial cell dysfunction. (A) Representative images of in vitro wound healing assay results. (B) Statistical analysis of in vitro wound healing. (C) Lower chamber fluorescence over time in permeability experiments. (D), (E) DCFH-DA staining fluorescence was used to detect intracellular ROS production and statistical analysis. (F), (G) Expression and statistical analysis of RTEF-1, VEGF, P2Y12, and INOS were investigated by WB. (H) Correlation between RTEF-1 and VEGF expression in GSE122952 dataset. All data are presented as mean ± SD, statistical analysis using one-way analysis of variance. ∗ P < 0.05, ∗∗ P < 0.01, ∗ versus Hypo group, # versus Si-RTEF-1-Sham group. Scale bars: 200 μm (B), 50 μm (E).

Article Snippet: The primary antibodies included GAPDH (Sequoia Golden Bridge, TA-08, China), RTEF-1 (Abcam, AB133533 , UK), VEGF (Cell Signaling Technology, 9698s, USA), INOS (Thermo Fisher Scientific, PA1-036, USA), and P2Y12 (Thermo Fisher Scientific, 702516, USA).

Techniques: In Vitro, Wound Healing Assay, Fluorescence, Permeability, Staining, Expressing

Journal: Neural Regeneration Research

Article Title: Prolonged intermittent theta burst stimulation restores the balance between A 2A R- and A 1 R-mediated adenosine signaling in the 6-hydroxidopamine model of Parkinson’s disease

doi: 10.4103/NRR.NRR-D-23-01542

Figure Lengend Snippet: Primary and secondary antibodies used in this study

Article Snippet: P2Y12R , Rabbit, polyclonal , 1:1000 WB , Alomone Labs, Jerusalem, Israel , AAR-012 , AB_2040074.

Techniques: Western Blot, Immunohistochemistry